If the H+ concentration is 0.00001 M, what is the OH- concentration?
1 answer:
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Answer:- 2.39 mL are required.
Solution:- It's a dilution problem and to solve this type of problems we use the dilution equation:
Where, and
are molarities of concentrated and diluted solutions and
and
are their respective volumes.
= 1.10M
= 5.00mM = 0.005M (since, mM stands for milli molar and M stands for molar. 1M = 1000mM)
= ?
= 525 mL
Let's plug in the given values in the formula:
So, 2.39 mL of 1.10M are needed to make 525 mL of 5.00mM solution.
Answer:
pH 9,8 is likely to work best for this separation
Explanation:
Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).
For lysine, PI is:
9,8
For arginine:
10,75
At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.
Thus, <em>pH 9,8 is likely to work best for this separation</em>
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I hope it helps!