What are hybrid orbitals?

1 answer:

3 0

Hybrid Orbitals: are used to describe the orbitals in covalently bonded atoms sp,sp2,sp3

You can find the hybridization by adding the number of bonded atoms and the number of lone pairs.

For example in BF3. The central atom (B) is bonded to three atoms. So the hybridization is sp2

In NH3, the central atom (N) is bonded to three atoms and has one lone pair. The hybridization is sp3

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a.) Asp and Lys

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Met will elute first from the column because it has less positively charged functional groups than Lys.

c.) Glu and Val

Glu will elute first from the column because it has more negativity functional groups than Lys and will be not be much retained by the -SO₃⁻ groups from the ion-exchange coloumn.

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e.) Ser and Ala

Ser will be eluted first from the column because Ala alkyl chain will be more attracted by the strongly hydrophobic backbone for the resin. Ser have an -OH group which will decrease the hydrophobicity of the alkyl chain and will not be so much retained on the column.