Derive the kinetic equations for Vmax and KM using the transit time and net rate constant method as discussed in class and follo
wing the attached handout. (Hint: solve for ET/v by summing the inverse net rate constants, then invert to obtain v/ET and put it in the form of kcatS/[KM S]). Remember that if a step is irreversible, the net rate constant for that step is just the rate constant for that step.
1 answer:
Answer:
Rate = vmax k3/k2+k3
Explanation:
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, is referred to as Vmax.
This is usually expressed as the Km ie. Michaelis constant of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.
Please kindly check attachment for the step by step solution of the given problem.
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(BELOW YOU CAN FIND ATTACHED THE IMAGE OF THE SITUATION)
Answer:
Explanation:
For this we're going to use conservation of mechanical energy because there are nor dissipative forces as friction. So, the change on mechanical energy (E) should be zero, that means:
(1)
With the initial kinetic energy,
the initial potential energy,
the final kinetic energy and
the final potential energy. Note that initialy the masses are at rest so
, when they are released the block 2 moves downward because m2>m1 and finally when the mass 2 reaches its maximum displacement the blocks will be instantly at rest so
. So, equation (1) becomes:
(2)
At initial moment all the potential energy is gravitational because the spring is not stretched so and at final moment we have potential gravitational energy and potential elastic energy so
, using this on (2)
(3)
Additional if we define the cero of potential gravitational energy as sketched on the figure below (See image attached), and we have by (3) :
(4)
Now when the block 1 moves a distance d upward the block 2 moves downward a distance d too (to maintain a constant length of the rope) and the spring stretches a distance d, so (4) is:
dividing both sides by d
, with k the constant of the spring and g the gravitational acceleration.
