Derive the kinetic equations for Vmax and KM using the transit time and net rate constant method as discussed in class and follo
wing the attached handout. (Hint: solve for ET/v by summing the inverse net rate constants, then invert to obtain v/ET and put it in the form of kcatS/[KM S]). Remember that if a step is irreversible, the net rate constant for that step is just the rate constant for that step.
1 answer:
Answer:
Rate = vmax k3/k2+k3
Explanation:
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, is referred to as Vmax.
This is usually expressed as the Km ie. Michaelis constant of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.
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Answer:
Due to equal pressure in all the direction at a particular level in a fluid medium (Pascal's Law)
Explanation:
We are not crushed by the weight of the atmosphere because atmosphere is a fluid and we are immersed into it. So, according to the Pascal's law the the pressure a each point in a horizontal level is equal in all the direction irrespective of the orientation of a body.
Variation of pressure in term of the height of a fluid medium is given as:
density of fluid
g = acceleration due to gravity
h = height of the free surface of the fluid from the immersed object.
- And atmosphere has very less variation of pressure with change in height as it is a rare medium fluid and so for a human height there is very negligible variation of pressure at the heat of a human with respect to his toe.