Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
it would be a covalent bond
Explanation:
carbon has 4 valence electrons, while chlorine has 7.
Continental plates are much thicker that Oceanic plates. At the convergent boundaries the continental plates are pushed upward and gain thickness. The rocks and geological layers are much older on continental plates than in the oceanic plates. The Continental plates are much less dense than the Oceanic plates.
Answer:
Answer is 'B' I think
Explanation:
Rutherford's Gold Foil Experiment proved the existence of a small massive center to atoms, which would later be known as the nucleus of an atom. Through previous experiments of shooting alpha particles, Rutherford knew they had considerable mass and speed.
<u>Answer:</u> The volume of stock solution needed is 90 mL
<u>Explanation:</u>
To calculate the molarity of the diluted solution, we use the equation:
where,
are the molarity and volume of the stock sulfuric acid solution
are the molarity and volume of diluted sulfuric acid solution
We are given:
Putting values in above equation, we get:
Hence, the volume of stock solution needed is 90 mL
