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diamong [38]
3 years ago
14

Predict the order of elution if you were to attempt to separate using column chromatography on silica gel and with a gradient el

uent system starting with petroleum ether and slowly increasing MTBE % over time.

Chemistry
1 answer:
Minchanka [31]3 years ago
6 0

Answer:

Follows this order: B=> A => C.

Explanation:

NB: kindly check the attachment for the diagram of compounds A, B and C.

Elution is a very important concept in chromatography separation techniques. It deals with the use of eluent in the removal of an adsobate from an adsorbent. The principle behind Elution is just about how polar the solvent is.

So, in this question Compound B will go with the Elution first because of its polarity. Compound B has lesser polarity as compared to Compounds A and B.

Compound A will then elutes second because of its polarity too as resonance increases its polarity.

Last, compound C elutes because it has the highest polarity which is caused by electronegative atoms.

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Your task is to create a buffered solution. You are provided with 0.10 M solutions of formic acid and sodium formate. Formic aci
Anton [14]

Answer:

15.2mL of the 0.10M sodium formate solution and 4.8mL of the 0.10M formic acid solution.

Explanation:

To find the pH of a buffer based on the concentration of the acid and conjugate base we must use Henderson-Hasselbalch equation:

pH = pKa + log [A⁻] / [HA]

<em>Where [A⁻] could be taken as moles of the sodium formate and [HA] moles of the formic acid</em>

<em />

4.25 = 3.75 + log [A⁻] / [HA]

0.5 = log [A⁻] / [HA]

3.162 = [A⁻] / [HA] <em>(1)</em>

<em></em>

As both solutions are 0.10M and you want to create 20mL of the buffer, the moles are:

0.10M  * 20x10⁻³L =

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Replacing (2) in (1):

3.162 = 2x10⁻³moles - [HA] / [HA]

3.162 [HA] = 2x10⁻³moles - [HA]

4.162[HA] = 2x10⁻³moles

[HA] = 4.805x10⁻⁴ moles

[A⁻] = 2x10⁻³moles - 4.805x10⁻⁴ moles = 1.5195x10⁻³moles

That means, to create the buffer you must add:

[A⁻] = 1.5195x10⁻³moles * (1L / 0.10mol) = 0.0152L =

<h3>15.2mL of the 0.10M sodium formate solution</h3>

[HA] = 4.805x10⁻⁴ moles * (1L / 0.10mol) = 0.0048L =

<h3>4.8mL of the 0.10M formic acid solution</h3>
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