Question

Suppose you are trying to separate three Proteins using Gel-Filtration chromatography. The sizes of each are given below:

Answer 1

Answer: The correct answer is Protein B.

Explanation:

Gel-filtration chromatography is a separation technique that is based on the size of the molecules in a compound. It is also known as size-exclusion chromatography in which the eluent (carrier) used is an aqueous solution.

The matrix that is used is a porous material. When the sample is inserted in the column, the smaller particles interact strongly with the matrix than the large ones. Thus, as the eluent is passed through the matrix, larger molecules come out first, and the smallest molecule comes out last.

Given sizes of the proteins:

Protein A: 1200 kDa

Protein B: 2000 kDa

Protein C: 800 kDa

As protein B has the largest size of all the given proteins, it will emerge out first from the column.

Hence, the correct answer is Protein B.