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2 years ago

Suppose you are trying to separate three Proteins using Gel-Filtration chromatography. The sizes of each are given below:

Chemistry

1 answer:

Damm [24]2 years ago

3 0

Answer: The correct answer is Protein B.

Explanation:

Gel-filtration chromatography is a separation technique that is based on the size of the molecules in a compound. It is also known as size-exclusion chromatography in which the eluent (carrier) used is an aqueous solution.

The matrix that is used is a porous material. When the sample is inserted in the column, the smaller particles interact strongly with the matrix than the large ones. Thus, as the eluent is passed through the matrix, larger molecules come out first, and the smallest molecule comes out last.

Given sizes of the proteins:

Protein A: 1200 kDa

Protein B: 2000 kDa

Protein C: 800 kDa

As protein B has the largest size of all the given proteins, it will emerge out first from the column.

Hence, the correct answer is Protein B.

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How many moles of carbon dioxide gas should be produced when 10.0 g of C2H6 are combusted at STP?

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0.665 moles of CO₂

Explanation:

The balance chemical equation for the combustion of Ethane is as follow:

2 C₂H₆ + 7 O₂ → 4 CO₂ + 6 H₂O

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Moles = Mass / M.Mass

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Step 2: Calculate Moles of CO₂ as;

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2 moles of C₂H₆ produced = 4 moles of CO₂

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0.3325 moles of C₂H₆ will produce = X moles of CO₂

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X = 0.3325 mol × 4 mol ÷ 2 mol

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