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2 years ago

Suppose you are trying to separate three Proteins using Gel-Filtration chromatography. The sizes of each are given below:

Chemistry

1 answer:

Damm [24]2 years ago

3 0

<u>Answer:</u> The correct answer is Protein B.

<u>Explanation:</u>

Gel-filtration chromatography is a separation technique that is based on the size of the molecules in a compound. It is also known as size-exclusion chromatography in which the eluent (carrier) used is an aqueous solution.

The matrix that is used is a porous material. When the sample is inserted in the column, the smaller particles interact strongly with the matrix than the large ones. Thus, as the eluent is passed through the matrix, larger molecules come out first, and the smallest molecule comes out last.

Given sizes of the proteins:

Protein A: 1200 kDa

Protein B: 2000 kDa

Protein C: 800 kDa

As protein B has the largest size of all the given proteins, it will emerge out first from the column.

Hence, the correct answer is Protein B.

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A standard solution of FeSCN2+ is prepared by combining 9.0 mL of 0.20 M Fe(NO3)3 with 1.0 mL of 0.0020 M KSCN . The standard so

Xelga [282]

Answer : The equilibrium concentration of $SCN^-$ in the trial solution is $4.58\times 10^{-8}M$

Explanation :

First we have to calculate the initial moles of $Fe^{3+}$ and $SCN^-$ .

$\text{Moles of }Fe^{3+}=\text{Concentration of }Fe^{3+}\times \text{Volume of solution}$

$\text{Moles of }Fe^{3+}=0.20M\times 9.0mL=1.8mmol$

and,

$\text{Moles of }SCN^-=\text{Concentration of }SCN^-\times \text{Volume of solution}$

$\text{Moles of }SCN^-=0.0020M\times 1.0mL=0.0020mmol$

The given balanced chemical reaction is,

$Fe^{3+}(aq)+SCN^-(aq)\rightleftharpoons FeSCN^{2+}(aq)$

Since 1 mole of $Fe^{3+}$ reacts with 1 mole of $SCN^-$ to give 1 mole of $FeSCN^{2+}$

The limiting reagent is, $SCN^-$

So, the number of moles of $FeSCN^{2+}$ = 0.0020 mmole

Now we have to calculate the concentration of $FeSCN^{2+}$ .

$\text{Concentration of }FeSCN^{2+}=\frac{0.0020mmol}{9.0mL+1.0mL}=0.00020M$

Using Beer-Lambert's law :

$A=\epsilon \times C\times l$

where,

A = absorbance of solution

C = concentration of solution

l = path length

$\epsilon$ = molar absorptivity coefficient

$\epsilon$ and l are same for stock solution and dilute solution. So,

$\epsilon l=\frac{A}{C}=\frac{0.480}{0.00020M}=2400M^{-1}$

For trial solution:

The equilibrium concentration of $SCN^-$ is,

$[SCN^-]_{eqm}=[SCN^-]_{initial}-[FeSCN^{2+}]$

$[SCN^-]_{initial}$ = 0.00050 M

Now calculate the $[FeSCN^{2+}]$ .

$C=\frac{A}{\epsilon l}=\frac{0.220}{2400M^{-1}}=9.17\times 10^{-5}M$

Now calculate the concentration of $SCN^-$ .

$[SCN^-]_{eqm}=[SCN^-]_{initial}-[FeSCN^{2+}]$

$[SCN^-]_{eqm}=(0.00050M)-(9.17\times 10^{-5}M)$

$[SCN^-]_{eqm}=4.58\times 10^{-8}M$

Therefore, the equilibrium concentration of $SCN^-$ in the trial solution is $4.58\times 10^{-8}M$

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3 years ago

you have a 500g block of butter at home. You found out that its volume its 555l. What is the density of the butter?

Ivenika [448]

Density will be 0.9009 g/L

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2 years ago

What are blocks of time in the geological time scale called?

Katena32 [7]

B is the answer , i got you

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3 years ago

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WILL GIVE BRAINLIEST, Which of the following would have the greatest amount of surface area?

Elodia [21]

Answer:

C. 1 cubic foot of loose sand

Explanation:

For many objects having equal volume , surface area will be maximum

of the object which has spherical shape .

But when a sphere is broken into tiny small spheres , total surface area of all the small spheres will be more than surface area of big sphere .

Hence among the given option , surface area of loose sand will have greatest surface area . Loose sand is equivalent to small spheres .

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3 years ago

Identify 3 items that are malleable

Stels [109]

Some examples of malleable materials are gold, silver, iron, aluminum, copper and tin.

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2 years ago