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julsineya [31]
3 years ago
7

Suppose you are trying to separate three Proteins using Gel-Filtration chromatography. The sizes of each are given below:

Chemistry
1 answer:
Damm [24]3 years ago
3 0

<u>Answer:</u> The correct answer is Protein B.

<u>Explanation:</u>

Gel-filtration chromatography is a separation technique that is based on the size of the molecules in a compound. It is also known as size-exclusion chromatography in which the eluent (carrier) used is an aqueous solution.

The matrix that is used is a porous material. When the sample is inserted in the column, the smaller particles interact strongly with the matrix than the large ones. Thus, as the eluent is passed through the matrix, larger molecules come out first, and the smallest molecule comes out last.

Given sizes of the proteins:

Protein A: 1200 kDa

Protein B: 2000 kDa

Protein C: 800 kDa

As protein B has the largest size of all the given proteins, it will emerge out first from the column.

Hence, the correct answer is Protein B.

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Which of the following concerning the effects of temperature and pressure on solubility is/are correct?
just olya [345]

Answer:

None of the options are correct.

Explanation:

1) when the temperature of the solution is increased the solubility of the gas in the liquid decreases , hence option 1 is incorrect.

2)The heat released by the dissolution of an ionic compound in water is heat of hydration of the compound and is independent of the initial temperature of the solution.

3) The solubility of a liquid in water is not affected significantly by the pressure changes in the system as gases only have a significant cahne in solubility with change in pressure.

5 0
3 years ago
You wish to generate 1.00 L of a 0.0750 M NaCl solution by diluting a more concentrated solution. What volume (in mL) of a 0.275
miss Akunina [59]

Taking into account the definition of dilution, you need to use a volume of 272.72 mL of a 0.275 M NaCl solution.

<h3>Dilution</h3>

When it is desired to prepare a less concentrated solution from a more concentrated one, it is called dilution.

Dilution is the process of reducing the concentration of solute in solution, which is accomplished by simply adding more solvent to the solution at the same amount of solute.

In a dilution the amount of solute does not change, but as more solvent is added, the concentration of the solute decreases, as the volume (and weight) of the solution increases.

A dilution is mathematically expressed as:

Ci×Vi = Cf×Vf

where

  • Ci: initial concentration
  • Vi: initial volume
  • Cf: final concentration
  • Vf: final volume

<h3>Initial volume</h3>

In this case, you know:

  • Ci= 0.275 M
  • Vi= ?
  • Cf= 0.0750 M
  • Vf= 1 L

Replacing in the definition of dilution:

0.275 M× Vi= 0.0750 M× 1 L

Solving:

Vi=(0.0750 M× 1 L)÷ 0.275 M

<u><em>Vi= 0.27272 L= 272.72 mL</em></u>

In summary, you need to use a volume of 272.72 mL of a 0.275 M NaCl solution.

Learn more about dilution:

brainly.com/question/6692004

brainly.com/question/16343005

brainly.com/question/24709069

#SPJ1

8 0
2 years ago
Why is it important for scientists to use the scientific method?
shtirl [24]
I believe the answer is A
3 0
3 years ago
Which of the following is true for a limiting reactant?
lbvjy [14]

Answer:

It is a reactant that produces smaller amount of product.

Explanation:

" The limiting reactant is a reactant that control the amount of product in chemical reaction because of its smaller amount"

Consider the example:

2 H₂ + O₂   →   2H₂O

two moles of hydrogen react with one moles of oxygen and produces two moles of water. when we take two mole of hydrogen and two mole of oxygen than again two mole of water is produced because hydrogen is limiting reactant and one mole of oxygen left un reacted.

Identification of limiting reactant:

Calculate the number of moles of reactant.

compare it with number of moles of product with help of balance chemical equation.

identify the reactant which produces less number of moles of product.

6 0
3 years ago
Which molecule make up proteins?
never [62]
The molecule that makes up proteins is amino acids.
4 0
3 years ago
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