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julsineya [31]
2 years ago
7

Suppose you are trying to separate three Proteins using Gel-Filtration chromatography. The sizes of each are given below:

Chemistry
1 answer:
Damm [24]2 years ago
3 0

<u>Answer:</u> The correct answer is Protein B.

<u>Explanation:</u>

Gel-filtration chromatography is a separation technique that is based on the size of the molecules in a compound. It is also known as size-exclusion chromatography in which the eluent (carrier) used is an aqueous solution.

The matrix that is used is a porous material. When the sample is inserted in the column, the smaller particles interact strongly with the matrix than the large ones. Thus, as the eluent is passed through the matrix, larger molecules come out first, and the smallest molecule comes out last.

Given sizes of the proteins:

Protein A: 1200 kDa

Protein B: 2000 kDa

Protein C: 800 kDa

As protein B has the largest size of all the given proteins, it will emerge out first from the column.

Hence, the correct answer is Protein B.

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<span>You can see that there are 2 moles of gas in the reactants and 3 moles of gas in the products. </span>

<span>1 moles of ideal gas occupies the same volume as 1 mole of any other ideal gas under the same conditions of temp and pressure. </span>

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<span>- the gas constant is different for different units of temp and pressure (see wikki link) in this case temp and pressure are constant, and we want to put the result in an equation that has Joules in it, so we select 8.314 JK^-1mol^-1) </span>
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