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<u>Answer:</u> The molecular weight of protein is
<u>Explanation:</u>
To calculate the concentration of solute, we use the equation for osmotic pressure, which is:
or,
where,
= Osmotic pressure of the solution = 0.0861 atm
i = Van't hoff factor = 1 (for non-electrolytes)
= mass of protein = 400 mg = 0.4 g (Conversion factor: 1 g = 1000 mg)
= molar mass of protein = ?
= Volume of solution = 5.00 mL
R = Gas constant =
T = temperature of the solution =
Putting values in above equation, we get:
Hence, the molecular weight of protein is