Answer:
C. Trp D. Phe E. Tyr
Explanation:
The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:
A = ∈ x c x l
where, A = Absorbance
∈ = Molar extinction co-efficient
c = Concentration of absorbing species i.e. protein
l = Path length of light
Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.
The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:
Amino acid Wavelength Molar extinction co-efficient (∈)
Tryptophan 282 nm 5690
Tyrosine 274 nm 1280
Phenylalanine 257 nm 570
In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.
The stock solution contains 10.5 moles of HCl per litre. A 5.5 litre solution of 2.5M HCl contains 5.5x2.5 = 13.75moles of HCl. Since every litre of stock solution provides 10.5M HCl, the amount of stock solution needed is 13.75/10.5 = 1.309L. Therefore you would dilute 1.309L of stock solution to 5.5L
Answer:
с
Explanation:
the first quantum number of an electron gives the information about the energy level the electron is in
