Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Main advantages of DDT are the insecticide property and the knoledge about its chemical synthesis.
Explanation:
At that time in 1939 it was discovered that DDT have insecticide properties. It is a useful property because it allows inhibition of insects populations in large areas. Killing insects will reduce the diseases transmitted by them as typhus and malaria. More over you prevent the destruction of the agricultural crops by the harmful insects.
However the synthesis of the molecule was known way back in 1874. From that time it was plenty of time in which chemistry knowledge evolved so the synthesis at kilograms scale was implemented. High quantities of DDT molecule become available for the market so that in 1945 was available as agricultural insecticide.
It was discovered that DDT have bad effects for human health and also over time some insects developed resistance and their were not affected anymore by the molecule.
You may find the chemical structure of DDT in the attached figure.
Lean more:
about DDT
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Answer:
How much heat energy required to convert following?
How much heat energy, in kilojoules, is required to convert 47.0 g of ice at -18.0 C to water at 25.0 C ?
Specific Heat of Ice - 2.09 j/g * c
This is how I did it and the answer is wrong...Please check and correct me
Q = m * Cice * Change in Temp
Q = (47.0 g)(2.09 J/g*c)(43) = 4222.6 J * 0.001 kj / j = 4.22 kj