Answer:
27 min
Explanation:
The kinetics of an enzyme-catalyzed reaction can be determined by the equation of Michaelis-Menten:
![v = \frac{vmax[S]}{Km + [S]}](https://tex.z-dn.net/?f=v%20%3D%20%5Cfrac%7Bvmax%5BS%5D%7D%7BKm%20%2B%20%5BS%5D%7D)
Where v is the velocity in the equilibrium, vmax is the maximum velocity of the reaction (which is directed proportionally of the amount of the enzyme), Km is the equilibrium constant and [S] is the concentration of the substrate.
So, initially, the velocity of the formation of the substrate is 12μmol/9min = 1.33 μmol/min
If Km is a thousand times smaller then [S], then
v = vmax[S]/[S]
v = vmax
vmax = 1.33 μmol/min
For the new experiment, with one-third of the enzyme, the maximum velocity must be one third too, so:
vmax = 1.33/3 = 0.443 μmol/min
Km will still be much smaller then [S], so
v = vmax
v = 0.443 μmol/min
For 12 μmol formed:
0.443 = 12/t
t = 12/0.443
t = 27 min
2-A
1-B
5-C
4-D
3-E
I hope this helped:)
Answer:
pH = 6.8124
Explanation:
We know pH decreases with increase in temperature.
At room temperature i.e. 25⁰c pH of pure water is equal to 7
We know
Kw = [H⁺][OH⁻]...............(1)
where Kw = water dissociation constant
At equilibrium [H⁺] = [OH⁻]
So at 37⁰c i.e body temperature Kw = 2.4 × 10⁻¹⁴
From equation (1)
[H⁺]² = 2.4 × 10⁻¹⁴
[H⁺] = √2.4 × 10⁻¹⁴
[H⁺] = 1.54 × 10⁻⁷
pH = - log[H⁺]
= - log{1.54 × 10⁻⁷}
= 6.812
Answer: A
Explanation:
Because I just answered it and it’s right. ( Forces between electron pairs push the atoms apart.
Answer:
300 mM
Explanation:
In order to solve this problem we need to calculate the line of best fit for those experimental values. The absorbance values go in the Y-axis while the concentration goes in the X-axis. We can calculate the linear fit using Microsoft Excel using the LINEST function (alternatively you can write the Y data in one column and X data in another one, then use that data to create a dispersion graph and finally add the line of best fit and its formula).
The <u>formula for the line of best fit for this set of data is</u>:
So now we <u>calculate the value of </u><u><em>x</em></u><u> when </u><u><em>y</em></u><u> is 1.50</u>:
