Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.

The stock concentration of NaCl (5 M ) is as follows:
.

The stock concentration of Imidazole (1 M ) is as follows:
.

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
The lighter components are able to rise higher in the column before they are cooled to their condensing temperature, allowing them to be removed at slightly higher levels.
I hope this helps
Answer:
a) 231.9 °C
b) 100% Sn
c) 327.5 °C
d) 100% Pb
Explanation:
This is a mixture of two solids with different fusion point:


<u>Given that Sn has a lower fusion temperature it will start to melt first at that temperature. </u>
So the first liquid phase forms at 231.9 °C and because Pb starts melting at a higher temperature, that phase's composition will be 100% Sn.
The mixture will be completely melted when you are a the higher melting temperature of all components (in this case Pb), so it will all in liquid phase at 327.5 °C.
At that temperature all Sn was already in liquid state and, therefore, the last solid's composition will be 100% Pb.
Answer: The author used the word "conversely" because the first statement he made is in CONTRAST to the second statement he made.
Explanation:
Matter is made up of atoms or molecules that are in constant motion. The motion of these tiny particles ( molecules) gives the object energy. The movement of these molecules depends on the state of matter which includes
--> GASEOUS STATE: Here, the particles are completely free to move and are always in motion.
--> LIQUID STATE: particles in this state slide by one another and are always in motion.
--> SOLID STATE: particles in this state are held tightly together but are always in motion.
Also, the molecules in motion are greatly affected by temperature changes. Increase in temperature will cause the particles in the liquid to move faster. Such is seen when soup is heated, the molecules travel faster than before. But the OPPOSITE is seen in an ice cube. This is because the ice cube is in solid state and of a lower temperature.
Answer:
The mass ratio of zinc to sulfide is 85:42.
2.5559 kg of Zn are in 3.82 kg of ZnS.
Explanation:
a) Mass of zinc sulfide = 254 g
Mass of zinc in a zinc sulfide sample = 170 g
Mass of sulfide in zinc sulfide sample = x
254 g = 170 g+ x
x = 84 g
The mass ratio of zinc to sulfide:

b) Mass of zincsulfide sample = 3.83 kg
The mass ratio of zinc to sulfide is 85:42.
Let the mass of zinc and sulfide be 85x and 42x respectively:
85 x+ 42 x=3.82 kg
x =0.03007 kg
Mass of an zinc= 85x=85 × 0.03007 kg= 2.5559 kg