Ammonium sulfate precipitation is used to separate proteins by function
However, it is one of the most commonly used methods for laboratory scale protein purification and fractionation. This method is also used to separate proteins by altering their solubility.
<h3>What is separation techniques?</h3>
Separation technique is a process or method used to the components of mixtures.
There are different types of separation technique which includes sublimation, precipitation, sieving, evaporation and many more.
Learn more about separation techniques:
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Answer:
56.9 mmoles of acetate are required in this buffer
Explanation:
To solve this, we can think in the Henderson Hasselbach equation:
pH = pKa + log ([CH₃COO⁻] / [CH₃COOH])
To make the buffer we know:
CH₃COOH + H₂O ⇄ CH₃COO⁻ + H₃O⁺ Ka
We know that Ka from acetic acid is: 1.8×10⁻⁵
pKa = - log Ka
pKa = 4.74
We replace data:
5.5 = 4.74 + log ([acetate] / 10 mmol)
5.5 - 4.74 = log ([acetate] / 10 mmol)
0.755 = log ([acetate] / 10 mmol)
10⁰'⁷⁵⁵ = ([acetate] / 10 mmol)
5.69 = ([acetate] / 10 mmol)
5.69 . 10 = [acetate] → 56.9 mmoles
Answer:
The molarity of the solution is 0,12 M.
Explanation:
We calculate the molarity, which is a concentration measure that indicates the moles of solute (in this case KOH) in 1000ml of solution (1 liter):
0,25 L solution----- 0,030 moles of KOH
1 L solution----x= (1 L solution x 0,030 moles of KOH)/0,25 L solution
x= 0,12 moles of KOH ---> <em>The solution is 0,12 M</em>
