As the building blocks of proteins , amino acids are linked to almost every life process, but they also have key roles as precursor compounds in many physiological processes.

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The absorbance (????)(A) of a solution is defined as ????=log10(????0????) A=log10⁡(I0I) where ????0I0 is the incident‑light int

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Answer:

The absorbance of the myoglobin solution across a 1 cm path is 0.84.

Explanation:

Beer-Lambert's law :

Formula used :

$A=\epsilon \times c\times l$

$A=\log \frac{I_o}{I}$

$\log \frac{I_o}{I}=\epsilon \times c\times l$

where,

A = absorbance of solution

c = concentration of solution

$\epsilon$ = Molar absorption coefficient

l = path length

$I_o$ = incident light

$I$ = transmitted light

Given :

l = 1 cm, c = 1 mg/mL , $\epsilon = 15,000 M^{-1}cm^{-1}$

Molar mass of myoglobin = 17.8 kDa = 17.8 kg/mol=17800 g/mol

(1 Da = 1 g/mol)

c = 1 mg /mL = ${1mg /mL}{\text{Molar mass of myoglobin}}$

$c = \frac{1 mg/mL}{ 17800 g/mol} = 5.6179\times 10^{-5} mol/L$

1 mg = 0.001 g, 1 mL = 0.001 L

$A= 15,000 M^{-1}cm^{-1}\times 5.6179\times 10^{-5} mol/L\times 1 cm$

$A=0.8426 \approx 0.84$

The absorbance of the myoglobin solution across a 1 cm path is 0.84.

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