Monoclonal antibodies are made by the fusion of cells of the immune system with B lymphocytes and myeloma cells.
<h3>What is Hybridoma technology?</h3>
- It is one of the best technologies that is used to produce the monoclonal antibody.
- In this hybridoma technology the B lymphocytes that are antibody producing are isolated from a source after the immunization with a specific antigen and then are fused with myeloma cell line to form hybrid cells that are also called as hybridoma cell lines.
- The hybridoma cell lines are then cultured in the laboratory with specific antigen and then the monoclonal antibodies are produced.
- This can be done in in-vivo or in-vitro condition.
- This method is preferred over all because the production of antibodies by this method is good as the antibodies produced are of high purity and highly sensitive and specific.
- These cell lines can also be preserved for a long time.
- Hybridoma technology has resulted in production of different varieties of monoclonal antibodies with specificity with specific antigens as the monoclonal antibodies are produced by single parental B cells.
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It will be transported into the cell through the process of endocytosis, which is a form of active transport. Active transport requires energy in the form of ATP. Hope this helps! :)
Diabetes type 1 is a chronic illness also referred to as juvenile diabetes or insulin-dependent diabetes.
<h3>
What is type-I Diabetes?</h3>
- The pancreas produces little or no insulin in this situation. Insulin is a hormone that the body utilizes to let glucose (sugar) into cells where it can be used to make energy.
- Type 1 diabetes may be brought on by a variety of factors, including genetics and some viruses. While type 1 diabetes typically first manifests in childhood or adolescence, it can also strike adults.
- There is still no cure for type 1 diabetes, despite much research. The goal of treatment is to prevent problems by controlling blood sugar levels with the use of insulin, food, and lifestyle changes.
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