Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
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The stock concentration of NaCl (5 M ) is as follows:
.

The stock concentration of Imidazole (1 M ) is as follows:
.

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
A small, green frog wearing a strawberry on its head as a hat
Answer: 54.94atm
Explanation: Please see attachment for explanation
Answer:
B. CO₂ and H₂O.
Explanation:
When any organic substance undergoes complete combustion, it produces carbon dioxide (CO₂) and water (H₂O).
In the case of methane, the balanced reaction is:
With the above information in mind the correct answer is option B. CO₂ and H₂O.
When the combustion is incomplete, carbon monoxide (CO) is produced as well.
Human activities have a tremendous impact on the carbon cycle. Burning fossil fuels, changing land use, and using limestone to make concrete all transfer significant quantities of carbon into the atmosphere. ... This extra carbon dioxide is lowering the ocean's pH, through a process called ocean acidification.