Question

Describe how thin layer chromatography is used in the isolation and extraction of lipids​

Answer 1

Thin layer chromatography(TLC) works with the principle of separation through adsorption.

It is used in the isolation and extraction of lipids through the following steps:

• apply the lipid samples spots in the bottom of the plate.
• also apply sample solution to the marked spot
• pour the mobile phase into the TLC chamber and use a moist filter paper to cover it. this is done to maintain equal humidity.
• then place the plate in the TLC chamber and close it with a lid.
• the plate is immersed into the solvent (mobile phase) for its development. this is done, keeping in mind that the sample spot should be above the solvent.
• once the sample spots are developed, they are removed and dried.
• this is later viewed using the UV light chamber to see the isolation of the lipid sample.

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