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hoa [83]
3 years ago
13

Describe how thin layer chromatography is used in the isolation and extraction of lipids​

Chemistry
1 answer:
BabaBlast [244]3 years ago
4 0

Thin layer chromatography(TLC) works with the principle of separation through adsorption.

It is used in the isolation and extraction of lipids through the following steps:

  • apply the lipid samples spots in the bottom of the plate.
  • also apply sample solution to the marked spot
  • pour the mobile phase into the TLC chamber and use a moist filter paper to cover it. this is done to maintain equal humidity.
  • then place the plate in the TLC chamber and close it with a lid.
  • the plate is immersed into the solvent (mobile phase) for its development. this is done, keeping in mind that the sample spot should be above the solvent.
  • once the sample spots are developed, they are removed and dried.
  • this is later viewed using the UV light chamber to see the isolation of the lipid sample.

Learn more here:

brainly.com/question/3137660

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