Matter is anything that has mass and volume
Answer:
2.down is hypotheses and 23.across is experiment
Explanation:
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a.) Asp and Lys
Asp will elute first from the column because it has less positively charged functional groups than Lys.
b.) Arg and Met
Met will elute first from the column because it has less positively charged functional groups than Lys.
c.) Glu and Val
Glu will elute first from the column because it has more negativity functional groups than Lys and will be not be much retained by the -SO₃⁻ groups from the ion-exchange coloumn.
d.) Gly and Val
Gly will elute first from the column because Lys have a longer alkyl chain which will be attracted by the strongly hydrophobic backbone for the resin.
e.) Ser and Ala
Ser will be eluted first from the column because Ala alkyl chain will be more attracted by the strongly hydrophobic backbone for the resin. Ser have an -OH group which will decrease the hydrophobicity of the alkyl chain and will not be so much retained on the column.
Answer:
The correct answer is: d. The pKa of the chosen buffer should be close to the optimal pH for the biochemical reaction.
Explanation:
The buffer resist or maintain the change in pH in case of Acid or basic addition to the solution. The buffer capacity should be within one or two pH units when compared to the optimal pH.
Thus it is important to select a buffer with pKa close to the optimum pH of the reaction because the ability for the buffer to maintain the pH is is great at the pH close to pKa.
Reactions happen instantly even if some are slower than others they all happen instantly so the answer would be A.