Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
is the isotopic notation of the atom
Explanation:
The isotope notation is:
<em>Where a is the mass number = Number of protons + Number of neutrons</em>
<em>b is atomic number = Number of protons</em>
The atomic number define the nature of the atom, the element with atomic number = 15 is phosphorus, P:
a = 15 protons + 16 neutrons = 31
b = 15
is the isotopic notation of the atom.
John Dalton
Although the concept of the atom dates back to the ideas of Democritus, the English meteorologist and chemist John Dalton formulated the first modern description of it as the fundamental building block of chemical structures.
It is harder to remove an electron from fluorine than from carbon because the size of the nuclear charge in fluorine is larger than that of carbon.
The energy required to remove an electron from an atom is called ionization energy.
The ionization energy largely depends on the size of the nuclear charge. The larger the size of the nuclear charge, the higher the ionization energy because it will be more difficult to remove an electron from the atom owing to increased electrostatic attraction between the nucleus and orbital electrons.
Since fluorine has a higher size of the nuclear charge than carbon. More energy is required to remove an electron from fluorine than from carbon leading to the observation that; it is harder to remove an electron from fluorine than from carbon.
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