Hey there!:
From the given data ;
Reaction volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )
Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:
[E]t in molar = g/L * mol/g
[E]t = 0.01 g/L * 1 / 45,000
[E]t = 2.22*10⁻⁷
Vmax = 0.758 umole/min/ per mL
= 758 mmole/L/min
=758000 mole/L/min => 758000 M
Therefore :
Kcat = Vmax/ [E]t
Kcat = 758000 / 2.2*10⁻⁷ M
Kcat = 3.41441 *10¹² / min
Kcat = 3.41441*10¹² / 60 per sec
Kcat = 5.7*10¹⁰ s⁻¹
Hence kcat of xyzase is 5.7*10¹⁰ s⁻¹
Hope that helps!
Answer:
90.46 well that's how I got the answer from my calculations
Substances that have a define size and shape and vibrating particles that are close together are called Solid.
Explanation:
There are three states of matter - solid, liquid and gas.
Lets talk about solid state in detail.
In solid particles are closely packed and so they have very less or no space to move which results in vibrating particles. This results in solid substances having a fixed shape and volume.
The particles have very little space in between them, so it is not easy to compress solid.
There is no free space for particles to slide over each other, which does not allow solids to flow.
Also, particles in solid have a regular arrangement, they are not randomly packed.
