Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
Learn more about the Nitrate reduction test with the help of the given link:
brainly.com/question/11181586
#SPJ4
Answer: 16700 Joules
Explanation:
The quantity of heat required to raise the temperature of a substance by one degree Celsius is called the specific heat capacity.
Q = Heat absorbed = ?
m = mass of sand = 2 kg
c = heat capacity = 
Initial temperature = 
= 
Final temperature= 
= 
Change in temperature ,
Putting in the values, we get:
16700 J of energy must be added to a 2-kilogram pile of it to increase its temperature from 40°C to 50°C
Hey there
The answer should be : Oxygen, fuel , heat
I hope that's help !
