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Answer:
The absorbance of the myoglobin solution across a 1 cm path is 0.84.
Explanation:
Beer-Lambert's law :
Formula used :
where,
A = absorbance of solution
c = concentration of solution
= Molar absorption coefficient
l = path length
= incident light
= transmitted light
Given :
l = 1 cm, c = 1 mg/mL ,
Molar mass of myoglobin = 17.8 kDa = 17.8 kg/mol=17800 g/mol
(1 Da = 1 g/mol)
c = 1 mg /mL =
1 mg = 0.001 g, 1 mL = 0.001 L
The absorbance of the myoglobin solution across a 1 cm path is 0.84.