Question

A solution was found to have a 15.6 % transmittance at 500 nm, its wavelength of maximum absorption, using a cell with a path length of 5.00 cm. Calculate the absorbance of the solution in a 1.00 cm cell at 500 nm. A. 0.161 B. 69.0 C. 0.0312 D. 4.04

Answer 1

For the absorbance of the solution in a 1.00 cm cell at 500 nm  is mathematically given as

A’ = 0.16138

What is the absorbance of the solution in a 1.00 cm cell at 500 nm?

Absorbance (A) 2 – log (%T) = 2 – log (15.6) = 0.8069

Generally, the equation for the Beer’s law is mathematically given as

A = ε*c*l

0.8069 = ε*c*(5.00 )

ε*c = 0.16138 cm-1

then for when ε*c is constant

l’ = 1.00

A’ = (0.16138 cm-1)*(1.00 cm)

A’ = 0.16138

In conclusion, the absorbance of the solution in a 1.00 cm cell at 500 nm is

A’ = 0.16138

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