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Svet_ta [14]
1 year ago
15

Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M in 50% aqueous

Chemistry
1 answer:
andreev551 [17]1 year ago
3 0

0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

<h3>What is dilution?</h3>

Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.

Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:

  • C1V1 = C2V2

where:

  • C1 = Initial concentration of enzyme
  • C2 = Final concentration of enzyme
  • V1 = Initial volume
  • V2 = Final volume

For the enzyme dilution;

C1 = 1 mg/mL

C2 = 1/50 mg/ml = 0.02 mg/ml

V= ?

V2 = 5 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

Learn more about dilution at: brainly.com/question/24881505

#SPJ1

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See Explanation

Explanation:

The question is incomplete; as the mixtures are not given.

However, I'll give a general explanation on how to go about it and I'll also give an example.

The percentage of a component in a mixture is calculated as:

\%C_E = \frac{E}{T} * 100\%

Where

E = Amount of element/component

T = Amount of all elements/components

Take for instance:

In (Ca(OH)_2)

The amount of all elements is: (i.e formula mass of (Ca(OH)_2))

T = 1 * Ca + 2 * H + 2 * O

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The amount of calcium is: (i.e formula mass of calcium)

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So, the percentage component of calcium is:

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\%C_E = \frac{4000}{74}\%

\%C_E = 54.05\%

The amount of hydrogen is:

E = 2 * H

E = 2 * 1

E = 2

So, the percentage component of hydrogen is:

\%C_E = \frac{E}{T} * 100\%

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Similarly, for oxygen:

The amount of oxygen is:

E = 2 * O

E = 2 * 16

E = 32

So, the percentage component of oxygen is:

\%C_E = \frac{E}{T} * 100\%

\%C_E = \frac{32}{74} * 100\%

\%C_E = \frac{3200}{74}\%

\%C_E = 43.24\%

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2 years ago
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