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Svet_ta [14]
1 year ago
15

Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M in 50% aqueous

Chemistry
1 answer:
andreev551 [17]1 year ago
3 0

0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

<h3>What is dilution?</h3>

Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.

Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:

  • C1V1 = C2V2

where:

  • C1 = Initial concentration of enzyme
  • C2 = Final concentration of enzyme
  • V1 = Initial volume
  • V2 = Final volume

For the enzyme dilution;

C1 = 1 mg/mL

C2 = 1/50 mg/ml = 0.02 mg/ml

V= ?

V2 = 5 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

Learn more about dilution at: brainly.com/question/24881505

#SPJ1

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frozen [14]

Answer:

oceanic formation is the right answer.

Explanation:

this os becoz they slide a past each other and do not rub against each other

4 0
3 years ago
I need help with homework
stellarik [79]

The equation that conforms with the particle model is 4C + 6O2 ----> 4CO2 + 2O2.

<h3>What is a chemical reaction?</h3>

The term chemical reaction has to do with the combination of substances to give products. We know that reactants and products must follow the law of conservation of mass.

Hence, the equation that conforms with the particle model is 4C + 6O2 ----> 4CO2 + 2O2.

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4 0
2 years ago
The decomposition of dinitrogen pentaoxide has an activation energy of 102 kj/mol and \delta ? h°rxn = + 55 kj/mol. what is the
posledela
A positive cahnge of enthalpy, ΔH rxn = + 55 kJ/mol, for the forward reaction means that the reaction is endothermic, i.e. the reactants absorb energy and the products are higher in energy.

Activation energy is the difference in the energy level of the reactants and the peak in the potential energy diagram (the energy of the transition state).

For an endothermic reaction, the products will be closer in energy to the transition state than what the reactans will be; so, the activation energy of the reversed reaction is lower than the activation energy of the forward reaction.

Activation energy of reverse and forward reactions is related by:

Activation energy of reverse rxn = Activation energy of forward rxn - ΔH rxn

=> Activiation energy of reverse rxn = 102 kJ/mol - 55 kJ/mol = 47 kJ/mol

Answer: 47 kJ/mol
8 0
3 years ago
B. The highly toxic gas phosgene is produced by the reaction CO(g) + Cl2(g) COCl2(g), ΔH = –108 kJ/mol. Use this reaction to ans
galben [10]

Answer:

I. Increasing pressure will allow more frequent successful collision between particles due to the particles being closer together.

II. Rate of reaction increases due to more products being made; as increased pressure favours the exothermic side of the equilibrium.

III. Increasing temperature provides particles lots of (Kinetic) energy, for more frequent successful collision due to the particles moving at a faster rate than before. However, favouring the endothermic side of the equilibrium due to lots of energy required to break and form new bonds.

IV. Rate of reaction increases due to increase temperature favouring both directions of the equilibrium - causing products to form faster.

Hope this helps!

6 0
2 years ago
How Many Moles Of HCl Need To Be Added To 150.0 ML Of 0.50 M NaZ To Have A Solution With A PH Of 6.50
Aleks04 [339]

The number of mole of HCl needed for the solution is 1.035×10¯³ mole

<h3>How to determine the pKa</h3>

We'll begin by calculating the pKa of the solution. This can be obtained as follow:

  • Equilibrium constant (Ka) = 2.3×10¯⁵
  • pKa =?

pKa = –Log Ka

pKa = –Log 2.3×10¯⁵

pKa = 4.64

<h3>How to determine the molarity of HCl </h3>
  • pKa = 4.64
  • pH = 6.5
  • Molarity of salt [NaZ] = 0.5 M
  • Molarity of HCl [HCl] =?

pH = pKa + Log[salt]/[acid]

6.5 = 4.64 + Log[0.5]/[HCl]

Collect like terms

6.5 – 4.64 = Log[0.5]/[HCl]

1.86 = Log[0.5]/[HCl]

Take the anti-log

0.5 / [HCl] = anti-log 1.86

0.5 / [HCl] = 72.44

Cross multiply

0.5 = [HCl] × 72.44

Divide both side by 72.44

[HCl] = 0.5 / 72.4

[HCl] = 0.0069 M

<h3>How to determine the mole of HCl </h3>
  • Molarity of HCl = 0.0069 M
  • Volume = 150 mL = 150 / 1000 = 0.15 L
  • Mole of HCl =?

Mole = Molarity x Volume

Mole of HCl = 0.0069 × 0.15

Mole of HCl = 1.035×10¯³ mole

<h3>Complete question</h3>

How many moles of HCl need to be added to 150.0 mL of 0.50 M NaZ to have a solution with a pH of 6.50? (Ka of HZ is 2.3 x 10 -5 .) Assume negligible volume of the HCl

Learn more about pH of buffer:

brainly.com/question/21881762

7 0
2 years ago
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