Question

3. A lab technician is preparing urea broth. It should be sterilized by filtration, he autoclaves it instead, just like he does for any other media. The high temperature of the autoclave causes the urea to break down. What color is the sterile urea broth when it comes out of the autoclave? but rstroedave tocave.

Answer 1

Urea get decomposed during autoclaving, because it is volatile. It is filter sterilized and added aseptically to your autoclave media.

Can urea be autoclaved?

• Urea get decomposed during autoclaving, because it is volatile.
• It is filter sterilized and added aseptically to your autoclave media.

What is autoclave sterilization?

• In medical and laboratory settings, an autoclave is used to sterilise waste and lab supplies.
• Heat is used in the autoclave sterilisation process to eliminate bacteria and spores. Pressurized steam provides the heat.

What is filter sterilization?

• By using filtering, it is possible to exclude organisms based on their size. There are many different kinds of filtration methods, but membrane filtration is utilised to sterilise a system.
• Contaminants that are larger than the pore size are captured by membrane filtration on the membrane's surface.

Learn more about autoclave media.

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