Answer:
3.6 × 10⁻⁵ M
Explanation:
Ergosterol has a maximum absorbance at λ = 282 nm. The absorbance of an analyte is related to its concentration through the Beer-Lambert's law.
A = ε × <em>l</em> × c
where,
A: absorbance
ε: molar absorptivity
<em>l</em>: optical path length
c; molar concentration
c = A / ε × <em>l </em>= 0.43 / (11,900 M⁻¹cm⁻¹) × 1.00 cm = 3.6 × 10⁻⁵ M
B. solvent
solute is the substance being dissolved
A solution has an absorbance of 0.2 with a path length of 1 cm. Given the molar absorptivity coefficient is 59 cm⁻¹ M⁻¹, the molarity is 0.003 M.
<h3>What does Beer-Lambert law state?</h3>
The Beer-Lambert law states that for a given material sample, path length and concentration of the sample are directly proportional to the absorbance of the light.
A solution has an absorbance of 0.2 with a path length of 1 cm. Given the molar absorptivity coefficient is 59 cm⁻¹ M⁻¹, we can calculate the molarity of the solution using the following expression.
A = ε × b × c
c = A / ε × b
c = 0.2 / (59 cm⁻¹ M⁻¹) × 1 cm = 0.003 M
where,
- A is the absorbance.
- ε is the path length.
- b is the molar absorptivity coefficient.
- c is the molar concentration.
A solution has an absorbance of 0.2 with a path length of 1 cm. Given the molar absorptivity coefficient is 59 cm⁻¹ M⁻¹, the molarity is 0.003 M.
Learn more about the Beer-Lambert law here: brainly.com/question/12975133
