Question

a solution with a transmittance of 0.44 is analyzed in a spectrophotometer with 6% stray light. calculate the absorbance reported by this defective instrument.

Answer 1

The absorbance reported by the defective instrument was 0.3933.

Absorbance A = - log₁₀ T

Tm = transmittance measured by spectrophotometer

Tm = 0.44

Absorbance reported in this equipment = -log₁₀ (0.44) = 0.35654

True absorbance can be calculated by true transmittance, Tm = T+S(α-T)

S = fraction of stray light = 6%= 6/100 = 0.06

α= 1, ideal case

T = true transmittance of the sample

Tm = T+S(α-T)

now, T= Tm-S/ 1-S = 0.44-0.06/ 1-0.06 = 0.404233

therefore, actual reading measured is A = -log₁₀ T = -log₁₀ (0.404233)

i.e; 0.3933

To know more about transmittance click here:

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