Consider an enzymatic reaction in which the initial concentration of substrate is low. If the amount of enzyme is held constant,
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Answer:
Use a ratio of 0.44 mol lactate to 1 mol of lactic acid
Explanation:
John could prepare a lactate buffer.
He can use the Henderson-Hasselbalch equation to find the acid/base ratio for the buffer.
He should use a ratio of 0.44 mol lactate to 1 mol of lactic acid.
For example, he could mix equal volumes of 0.044 mol·L⁻¹ lactate and 0.1 mol·L⁻¹ lactic acid.