A foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction.
<h3>How to explain the reaction?</h3>
With the aid of two enzymes, ligase and restriction enzymes, a foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction. Each enzyme detects a target DNA sequence and cuts it nearby, while ligase aids in connecting the DNA. When two bits of DNA have complimentary bases, it facilitates their joining.
Plasmid and the insert fragment are both present in the microfuge tube, and they both have compatible sticky ends. However, the ligase has been denatured and is no longer active because the prior student left it outside rather than freezing it; despite this, we had already put the ligase into the tube. Ligase aids in binding the plasmid and insert fragments together, but because it is denatured in this instance, it will no longer be able to do so. As a result, no transformation process will take place. And since ligase links DNA fragments together by catalyzing the development of connections between the nearby nucleotides, the two fragments will not be able to unite.
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Answer:
For the first question, to determine the total number of molecules of nitrogen dioxide, first make use of the molar mass of the nonpolar compound and then use that to find the total number of moles and then subsequently after make use of the ratio for the Avogadro's number to determine the total number of molecules of this compound.
For the final question, do the inverse, where we make use of the molecules of the compound and then use Avogadro's number to determine the moles of the compound and then use the same molar mass of the compound to determine the grams of the Nitrogen Dioxide.
Answer:
The answer is 213121119.94. Please mark me brainliest if I helped.
