Question

Given that the concentration of bovine carbonic anhydrase is 3.3 pmol ⋅ L − 1 and R max ( V max ) = 222 μmol ⋅ L − 1 ⋅ s − 1 , determine the turnover number of the enzyme molecule bovine carbonic anhydrase, which has a single active site.

Answer 1

Answer:

The turnover number of the enzyme molecule bovine carbonic anhydrase = 67,272,727.27 s^–1.

Explanation:

Given:

The concentration of bovine carbonic anhydrase = total enzyme concentration = Et = 3.3 pmol⋅L^–1 = 3.3 × 10^–12 mol.L^–1

The maximum rate of reaction = Rmax (Vmax) = 222 μmol⋅L^–1⋅s^–1 = 222 × 10^–6 mol.L^–1⋅s^–1

The formula for the turnover number of an enzyme (kcat, or catalytic rate constant) = Rmax ÷ Et = 222 × 10^–6 mol.L^–1⋅s^–1 ÷ 3.3 × 10^–12 mol.L^–1 = 67,272,727.27 s^–1

Therefore, the turnover number of the enzyme molecule bovine carbonic anhydrase = 67,272,727.27 s^–1