Answer:
C. Trp D. Phe E. Tyr
Explanation:
The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:
A = ∈ x c x l
where, A = Absorbance
∈ = Molar extinction co-efficient
c = Concentration of absorbing species i.e. protein
l = Path length of light
Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.
The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:
Amino acid Wavelength Molar extinction co-efficient (∈)
Tryptophan 282 nm 5690
Tyrosine 274 nm 1280
Phenylalanine 257 nm 570
In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.
There are 1.93 x 10²⁴ particles
<h3>Further explanation</h3>
Given
3.2 moles of Neon gas
Required
Number of particles
Solution
The mole is the number of particles(molecules, atoms, ions) contained in a substance
<em>1 mol = 6.02.10²³ particles
</em>
Can be formulated
N=n x No
N = number of particles
n = mol
No = Avogadro's = 6.02.10²³
So the number of particles for 3.2 moles :
N = 3.2 x 6.02.10²³
N = 1.93 x 10²⁴
or
we can describe it using Avogadro's number conversion factor

Answer: Diffusion is the movement of particles from a high to low particle concentration, while osmosis is the movement of water from a high to a low water concentration.
Explanation:
8.50 moles is equal to 5.1187×10²⁴ atoms of Ca.
<u>Explanation:</u>
We have to multiply the moles of Ca by the Avogadro's number:
= 6.022×10²³
So the number of atoms:
= 8.5 moles × 6.022×10²³atoms / mol
= 5.1187×10²⁴ atoms
Hence the 8.50 moles is equal to 5.1187×10²⁴ atoms of Ca.