0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
Learn more about dilution at: brainly.com/question/24881505
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Answer:
7,01 g of indole-3-acetic acid
Explanation:
The milimolar concentration (mM) is defined as the ratio of milimoles per Liter of solution. 400mM means 400mmoles / L that is the same of <em>0,4mol / L</em>
100mL are <em>0,1L</em>. Using these values:
0,1L × (0,4mol / L ) = 0,04moles of indole-3-acetic acid.
As the MW of the molecule is 175,2 g/mol:
0,04mol × (175,2g / mol) = <em>7,01 g of indole-3-acetic acid</em>
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Thus, <em>you need 7,01 g of indole-3-acetic acid to generate your solution</em>.
I hope it helps!
To know the number of atoms, it will be necessary to use the formula of avogadro. We calculate the number of moles then we can calculate the number of avogadro.
The molar mass of:
Phosphorus = 31g / mol
Mercury = 200 g / mol
Bismuth = 209 g / mol
Strontium = 87 g / mol.
The number of avogadro (N) is 6.023 10 ^ 23
n = m (mass) / M (molar mass)
number of atoms = n. N = m / M . N
number of phosphorus atoms = 5.14/31 . 6.023 10^23 = 9.98 10 ^ 22 atoms
Number of atoms of mercury = 2.16/200 . 6.023 10^23 = 6.5 10 ^ 21 atoms
Number of bismuth atoms = 1.8/209 . 6.023 10^23 = 5.18 10 ^ 21 atoms
Number of strontium atoms = 8.8 x 10-2 /87 . 6.023 10^23 = 6.09 10 ^ 20 atoms
<span> endothermic is the answer
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