Here we have to write a simple equation which describes the action of the enzyme catalase.
The equation is: The concentration of the complex [ES] =
Let us consider an enzyme catalyses reaction E + S ⇄ ES → E + P
Where E, S, ES and P are enzyme, substrate, complex and product respectively.
The concentration of the complex [ES] = , where
is the Michaelis constant.
[E]₀ and [S] is the initial concentration of enzyme and concentration of substrate respectively.
a.) Asp and Lys
Asp will elute first from the column because it has less positively charged functional groups than Lys.
b.) Arg and Met
Met will elute first from the column because it has less positively charged functional groups than Lys.
c.) Glu and Val
Glu will elute first from the column because it has more negativity functional groups than Lys and will be not be much retained by the -SO₃⁻ groups from the ion-exchange coloumn.
d.) Gly and Val
Gly will elute first from the column because Lys have a longer alkyl chain which will be attracted by the strongly hydrophobic backbone for the resin.
e.) Ser and Ala
Ser will be eluted first from the column because Ala alkyl chain will be more attracted by the strongly hydrophobic backbone for the resin. Ser have an -OH group which will decrease the hydrophobicity of the alkyl chain and will not be so much retained on the column.
