Question

Phosphate buffer solution is purchased as a(n) 10x stock solution. The solution is more concentrated than the working stock. If tasked with making 75 mL of a 1x solution of PBS, how many mL of the stock solution would be needed

Answer 1

Answer:

v₁ = 7.5mL

Explanation:

using the formula

m₁v₁=m₂v₂

given m₁=10x m₂=1x v₁=? v₂=75mL

10x × v₁ = 1x × 75mL

v₁ = 1x × 75mL / 10x

v₁ = 7.5mL

So, 7.5mL of 10x PBS should be added with 67.5mL of water