At the half equivalence point [HA] = [A-] and pH = pKa
<span>if Ka is 5.2e-5 then pKa = pH = 4.28</span>
Answer:
The mass was there all along, it was just in the air. The weight of the oxygen from the air is not weighed in the beginning, only at the end as part of the product, making it seem like there is a total mass change.
Answer:
Taq polymerase is a thermostable which states that it can even work at higher temperature. The main function of using this enzyme is that it is used to amplify the DNA which will help in producing ample amount of DNA sample.
The enzyme activity is temperature dependent. The denaturation and annealing steps of the PCR occurs at very high temperature.
Any other enzyme used at such an high temperature would have decreased the enzyme activity and the procedure would not be completed.