Answer:
THE LENGTH OF THE AIR COLUMN IS 9.5 CM
Explanation:
Taking the atmospheric pressure to be 760 mmHg;
When the capillary tube is held horizontally, the pressure of the tube is 760 mmHg
when the capillary tube is held vertically, the pressure increases by 4 cm = 40 mm
The new pressure of the tube is hence, 760 + 40 mmHg = 800 mmHg
Using the pressure forlmula;
P1 V1 = P2 V2
P1 A1 L1 = P2 A2 L2
where A1 and A2 is the area of the capillary tube and it is equal, it cancels out.
P1 l1 = P2 l2
l2 = P1 l1 / P2
l2 = 760 * 10 / 800
l2 = 9.5 cm
The length of the air in the tube is 9.5 cm.
Quantity of K2S m = 0.105 m
Number of ions i = 2(K) + 1(S) = 3
Freezing point depression constant of water Kf = 1.86
delta T = i x m x Kf = 3 x 0.105 x 1.86 = 0.586
Freezing point = 0 - 0.586 = 0.586 C
Boiling point constant of water Kb = 0.512
delta T = i x m x Kb = 3 x 0.105 x 0.512 = 0.161
Boiling point = 100 + 0.161 = 100.161 C
A foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction.
<h3>How to explain the reaction?</h3>
With the aid of two enzymes, ligase and restriction enzymes, a foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction. Each enzyme detects a target DNA sequence and cuts it nearby, while ligase aids in connecting the DNA. When two bits of DNA have complimentary bases, it facilitates their joining.
Plasmid and the insert fragment are both present in the microfuge tube, and they both have compatible sticky ends. However, the ligase has been denatured and is no longer active because the prior student left it outside rather than freezing it; despite this, we had already put the ligase into the tube. Ligase aids in binding the plasmid and insert fragments together, but because it is denatured in this instance, it will no longer be able to do so. As a result, no transformation process will take place. And since ligase links DNA fragments together by catalyzing the development of connections between the nearby nucleotides, the two fragments will not be able to unite.
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