Question

3A. A student slurry packed a silica gel column for the separation of 2-nitroanaline and 4-nitroanaline as described in the experiment given. During packing, they are not paying close enough attention and allow the top of the silica gel to run dry causing cracking within the silica gel. The student continues with the experiment and applies the mixture of isomers to the chromatography column. While the yellow band of the sample is being eluted, the student observes that there isn’t a clear separation with two separate yellow bands for each isomer and the band does not appear to be moving in a level band. Instead the band is slightly slanted What caused the poor separation of the isomers? What could the student do differently in the future to improve the results of the experiment?

Answer 1

Answer:

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Explanation: