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pashok25 [27]
3 years ago
13

Why must the spectrophotometer cell be removed from the instrument between readings?

Chemistry
1 answer:
Natali [406]3 years ago
5 0

Spectrophotometric cell or a cuvette is made of quartz for UV spectrophotometers. These cuvettes are used as sample holders for the spectrophotometric determination of the analytes. The material that makes up the cuvette and the condition of the cuvette is to be taken care of in order to avoid erroneous absorbance readings. The sample holder or the cuvette must be removed from the spectrophotometer in between two successive readings. This is to ensure that the light sensing detector of the instrument is not affected.

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How many oxygen atoms are there in 582 grams of caffeine (CH10N402, 194 g/mol)? Avogadro's Number: 1 mole = 6.02 x 1023 species
Sergeu [11.5K]

Answer:

3.61 * 10 ²⁴atoms.

Explanation:

Moles is denoted by given mass divided by the molecular mass ,

Hence ,

n = w / m

n = moles ,

w = given mass ,

m = molecular mass .

From the question ,

w = 582 g

m = 194 g/mol

The number of moles can be calculated from the above formula , and substituting the respective values ,

n = w / m  = 582 g / 194 g/mol  =  3 mol

In the molecular formula of caffeine ,

<u>In 1 mole of caffeine their are - 2 moles of Oxygen. </u>

Therefore , in  3 moles of Caffeine there will be 6 moles of oxygen.

As well know ,

one mole of any substance contains 6.023*10²³ atoms,

Therefore , in  6 mol of oxygen =  6 * 6.023*10²³ atoms  = 3.61 * 10 ²⁴atoms.

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