Answer:
The correct/closest option is b
Explanation:
Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.
A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.
For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.
G║AATTC
CTTAA║G
And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.
GGG║CCC
CCC║GGG
Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.
Answer: The net force in every bolt is 44.9 kip
Explanation:
Given that;
External load applied = 245 kip
number of bolts n = 10
External Load shared by each bolt (P_E) = 245/10 = 24.5 kip
spring constant of the bolt Kb = 0.4 Mlb/in
spring constant of members Kc = 1.6 Mlb/in
combined stiffness factor C = Kb / (kb+kc) = 0.4 / ( 0.4 + 1.6) = 0.4 / 2 = 0.2 Mlb/in
Initial pre load Pi = 40 kip
now for Bolts; both pre load Pi and external load P_E are tensile in nature, therefore we add both of them
External Load on each bolt P_Eb = C × PE = 0.2 × 24.5 = 4.9 kip
So Total net Force on each bolt Fb = P_Eb + Pi
Fb = 4.9 kip + 40 kip
Fb = 44.9 kip
Therefore the net force in every bolt is 44.9 kip
A safety device called a cotter pin. The cotter pin fits through a hole in the bolt or part. This keeps the nut from turning and possibly coming off.
Answer: double click at the top of the page. Or you can also go to home file and click add heading.
Explanation:
