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3 years ago

During genetic engineering, how do Restriction enzymes know what base pairs to act on?

Engineering

1 answer:

MaRussiya [10]3 years ago

6 0

Answer:

The correct/closest option is b

Explanation:

Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.

A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.

For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.

G║AATTC

CTTAA║G

And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.

GGG║CCC

CCC║GGG

Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.

You might be interested in

The function below takes a single parameter, a list of numbers called number_list. Complete the function to return a string of t

makkiz [27]

Answer:

The solution code is written in Python:

def convertCSV(number_list):
str_list = []
for num in number_list:
str_list.append(str(num))
return ",".join(str_list)
result = convertCSV([22,33,44])
print(result)

Explanation:

Firstly, create a function "convertCSV" with one parameter "number_list". (Line 1)

Next, create an empty list and assign it to a new variable str_list. (Line 2)

Use for-loop to iterate through all the number in the number_list.(Line 4). Within the loop, each number is converted to a string using the Python built-in function str() and then use the list append method to add the string version of the number to str_list.

Use Python string join() method to join all the elements in the str_list as a single string. The "," is used as a separator between the elements (Line 7) . At the end return the string as an output.

We can test the function by calling the function and passing [22,33,34] as an argument and we shall see "22,33,44" is printed as an output. (Line 9 - 10)

6 0

3 years ago

For some transformation having kinetics that obey the Avrami equation, the parameter n is known to have a value of 2. If, after

kotegsom [21]

This question is incomplete, the complete question is;

For some transformation having kinetics that obey the Avrami equation, the parameter n is known to have a value of 2. If, after 100 s, the reaction is 40% complete, how long (total time in seconds) will it take the transformation to go to 95% completion

y = 1 - exp( -ktⁿ )

Answer: the time required for 95% transformation is 242.17 s

Explanation:

First, we calculate the value of k which is the dependent variable in Avrami equation

y = 1 - exp( -ktⁿ )

exp( -ktⁿ ) = 1 - y

-ktⁿ = In( 1 - y )

k = - In( 1 - y ) / tⁿ

now given that; n = 2, y = 40% = 0.40, and t = 100 s

we substitute

k = - In( 1 - 0.40 ) / 100²

k = - In(0.60) / 10000

k = 0.5108 / 10000

k = 0.00005108 ≈ 5.108 × 10⁻⁵

Now calculate the time required for 95% transformation

tⁿ = - In( 1 - y ) / k

t = [- In( 1 - y ) / k ]^1/n

n = 2, y = 95% = 0.95 and k = 5.108 × 10⁻⁵

we substitute our values

t = [- In( 1 - 0.95 ) / 5.108 × 10⁻⁵ ]^1/2

t = [2.9957 / 5.108 × 10⁻⁵]^1/2

t = [ 58647.22 ]^1/2

t = 242.17 s

Therefore the time required for 95% transformation is 242.17 s

8 0

3 years ago

The results of a _________ test will determine if there is suitable drainage and the size of the drain field that will be requir

topjm [15]

Answer:

The results of a percolation test will determine if there is suitable drainage and the size of the drain field that will be required for a septic system.

7 0

2 years ago

A(n) is a detailed, structured diagram or drawing.

monitta

Answer:

Schematics

Explanation:

A schematic is a detailed structured diagram or drawing. It employs illustrations to help the viewer understand detailed information on the machine or object being described. Its main aim is not to help the observer know what the object looks like physically. It is rather aimed at helping the viewer know how the machine works. This is achieved by only including key and important details to the drawing.

It is most times used in the blueprint and user guides of machines and gadgets used in the home to help users know how these things work so that they can do little fixings should there be such needs.

6 0

3 years ago

Plot the function for . Notice that the function has two vertical asymptotes. Plot the function by dividing the domain of x into

elena-s [515]

This is a very very difficult one for me, let me get back to you with the proper answer.

8 0

3 years ago