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shepuryov [24]
3 years ago
10

During genetic engineering, how do Restriction enzymes know what base pairs to act on?

Engineering
1 answer:
MaRussiya [10]3 years ago
6 0

Answer:

The correct/closest option is b

Explanation:

Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.

A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.

For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.

G║AATTC

CTTAA║G

And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.

GGG║CCC

CCC║GGG

Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.

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Good night. I need to go to bed. Byeeeeeeeeeeeee.​
dimaraw [331]

Answer:

BYEEEEEEEEEEEE3EEEEEEEEEE

Explanation:

dawg

8 0
3 years ago
2. The following segment of carotid artery has an inlet velocity of 50 cm/s (diameter of 15 mm). The outlet has a diameter of 11
ahrayia [7]

This question is incomplete, the missing diagram is uploaded along this answer below.

Answer:

the forces required to keep the artery in place is 1.65 N

Explanation:

Given the data in the question;

Inlet velocity V₁ = 50 cm/s = 0.5 m/s

diameter d₁ = 15 mm = 0.015 m

radius r₁ = 0.0075 m

diameter d₂ = 11 mm = 0.011 m

radius r₂ = 0.0055 m

A₁ = πr² = 3.14( 0.0075 )² =  1.76625 × 10⁻⁴ m²

A₂ = πr² = 3.14( 0.0055 )² =  9.4985 × 10⁻⁵ m²

pressure at inlet P₁ = 110 mm of Hg = 14665.5 pascal

pressure at outlet P₂ = 95 mm of Hg = 12665.6 pascal

Inlet volumetric flowrate = A₁V₁ = 1.76625 × 10⁻⁴ × 0.5 = 8.83125 × 10⁻⁵ m³/s

given that; blood density is 1050 kg/m³

mass going in m' = 8.83125 × 10⁻⁵ m³/s × 1050 kg/m³ = 0.092728 kg/s

Now, using continuity equation

A₁V₁ = A₂V₂

V₂ = A₁V₁ / A₂ = (d₁/d₂)² × V₁

we substitute

V₂ =  (0.015 / 0.011 )² × 0.5

V₂ = 0.92975 m/s

from the diagram, force balance in x-direction;

0 - P₂A₂ × cos(60°) + Rₓ = m'( V₂cos(60°) - 0 )    

so we substitute in our values

0 - (12665.6 × 9.4985 × 10⁻⁵)  × cos(60°) + Rₓ = 0.092728( 0.92975 cos(60°) - 0 )    

0 - 0.6014925 + Rₓ =  0.043106929 - 0

Rₓ = 0.043106929 + 0.6014925

Rₓ = 0.6446 N

Also, we do the same force balance in y-direction;

P₁A₁ - P₂A₂ × sin(60°) + R_y = m'( V₂sin(60°) - 0.5 )  

we substitute

⇒ (14665.5 × 1.76625 × 10⁻⁴) - (12665.6 × 9.4985 × 10⁻⁵) × sin(60°) + R_y = 0.092728( 0.92975sin(60°) - 0.5 )

⇒ 1.5484 + R_y = 0.092728( 0.305187 )

⇒ 1.5484 + R_y = 0.028299    

R_y = 0.028299 - 1.5484

R_y = -1.52 N

Hence reaction force required will be;

R = √( Rₓ² + R_y² )

we substitute

R = √( (0.6446)² + (-1.52)² )

R = √( 0.41550916 + 2.3104 )

R = √( 2.72590916 )

R = 1.65 N

Therefore, the forces required to keep the artery in place is 1.65 N

 

7 0
2 years ago
A rectangular open box, 25 ft by 10 ft in plan and 12 ft deep weighs 40 tons. Sufficient amount of stones is placed in the box a
dimulka [17.4K]

Answer:

44.95 tonnes

Explanation:

According to principle of buoyancy the object will just sink when it's weight is more than the weight of the liquid it displaces

It is given that empty weight of box = 40 tons

Let the mass of the stones to be placed be = M tonnes

Thus the combined mass of box and stones = (40+M) tonnes..........(i)

Since the box will displace water equal to it's volume V we have volume of box = 25ft*10ft*12ft= 3000ft^{3}

Volume= 84.95m^{3}

Since 1ft^{3} =0.028m^{3}

Now the weight of water displaced = Weight =\rho \times Volumewhererho is density of water = 1000kg/m^{3}

Thus weight of liquid displaced = \frac{84.95X1000}{1000}tonnes=84.95 tonnes..................(ii)

Equating i and ii we get

40 + M = 84.95

thus Mass of stones = 44.95 tonnes

3 0
3 years ago
\o you sell them?” "Fivepence farthing for one—Twopence for two,” the Sheep replied. "Then two are cheaper than one?” Alice said
NemiM [27]

Answer:

unusual and strange

Explanation:

3 0
3 years ago
Write a class named FBoard for playing a game, where player x is trying to get her piece to row 7 and player o is trying to make
OverLord2011 [107]

Answer:

12345=V

Explanation:

7 0
3 years ago
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