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3 years ago

During genetic engineering, how do Restriction enzymes know what base pairs to act on?

Engineering

1 answer:

MaRussiya [10]3 years ago

6 0

Answer:

The correct/closest option is b

Explanation:

Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.

A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.

For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.

G║AATTC

CTTAA║G

And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.

GGG║CCC

CCC║GGG

Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.

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How do you identify all sensors, functions, and where we can use them?

Alex17521 [72]

Sensor/Detectors/Transducers are electrical, opto-electrical, or electronic devices composed of specialty electronics or otherwise sensitive materials, for determining if there is a presence of a particular entity or function. Many vehicles including cars, trains, buses etc. all use sensors to monitor oil temperature and pressure, throttle and steering systems and so many more aspects.

7 0

3 years ago

At a point on the free surface of a stressed body, the normal stresses are 20 ksi (T) on a vertical plane and 30 ksi (C) on a ho

victus00 [196]

Answer:

The principal stresses are σp1 = 27 ksi, σp2 = -37 ksi and the shear stress is zero

Explanation:

The expression for the maximum shear stress is given:

$\tau _{M} =\sqrt{(\frac{\sigma _{x}^{2}-\sigma _{y}^{2} }{2})^{2}+\tau _{xy}^{2} }$

Where

σx = stress in vertical plane = 20 ksi

σy = stress in horizontal plane = -30 ksi

τM = 32 ksi

Replacing:

$32=\sqrt{(\frac{20-(-30)}{2} )^{2} +\tau _{xy}^{2} }$

Solving for τxy:

τxy = ±19.98 ksi

The principal stress is:

$\sigma _{x}+\sigma _{y} =\sigma _{p1}+\sigma _{p2}$

Where

σp1 = 20 ksi

σp2 = -30 ksi

$\sigma _{p1} +\sigma _{p2}=-10 ksi$ (equation 1)

$\tau _{M} =\frac{\sigma _{p1}-\sigma _{p2}}{2} \\\sigma _{p1}-\sigma _{p2}=2\tau _{M}\\\sigma _{p1}-\sigma _{p2}=32*2=64ksi$ equation 2

Solving both equations:

σp1 = 27 ksi

σp2 = -37 ksi

The shear stress on the vertical plane is zero

4 0

4 years ago

While discussing what affects the amount of pressure exerted by the brakes: Technician A says that the shorter the line, the mor

harina [27]

Answer:

Only Technician B is right.

Explanation:

The cylindrical braking system for a car works through the mode of pressure transmission, that is, the pressure applied to the brake pedals, is transmitted to the brake pad through the cylindrical piston.

Pressure applied on the pedal, P(pedal) = P(pad)

And the Pressure is the applied force/area for either pad or pedal. That is, P(pad) = Force(pad)/A(pad) & P(pedal) = F(pedal)/A(pedal)

If the area of piston increases, A(pad) increases and the P(pad) drops, Meaning, the pressure transmitted to the pad reduces. And for most cars, there's a pressure limit for the braking system to work.

If the A(pad) increases, P(pad) decreases and the braking force applied has to increase, to counter balance the dropping pressure and raise it.

This whole setup does not depend on the length of the braking lines; it only depends on the applied force and cross sectional Area (size) of the piston.

5 0

3 years ago

When designing a car that runs on wind or Air car . can you tell me the details for the following points Compressed Air Engine:

BabaBlast [244]

Answer:

The crack and connecting rod is used in the design of car.This mechanism is known as slider -crank mechanism.

Components:

1.Inlet tube

2. Wheel

3. Exhaust

4. Engine

5.Air tank

6.Pressure gauge

7.Stand

8. Gate valve

The efficiency of air engine is less as compare to efficiency of electric engine and this is not ecofriendly because it produce green house gases.These gases affect the environment.

it can run around 722 km when it is full charge.

5 0

3 years ago

A cylindrical drill with radius 4 is used to bore a hole through the center of a sphere of radius 5. Find the volume of the ring

ANTONII [103]

Answer:

The volume of the ring shaped solid that remains is 21 unit^3.

Explanation:

The total volume of the sphere is given as:

Volume of Sphere = (4/3)πr^3

where, r = radius of sphere

Volume of Sphere = (4/3)(π)(5)^3

Volume of Sphere = 523.6 unit^3

Now, we find the volume of sphere removed by the drill:

Volume removed = (Cross-sectional Area of drill)(Diameter of Sphere)

Volume removed = (πr²)(D)

where, r = radius of drill = 4

D = diameter of sphere = 2*5 = 10

Therefore,

Volume removed = (π)(4)²(10)

Volume removed = 502.6 unit^3

Therefore, the volume of ring shaped solid that remains will be the difference between the total volume of sphere, and the volume removed.

Volume of Ring = Volume of Sphere - Volume removed

Volume of Ring = 523.6 - 502.6

<u>Volume of Ring = 21 unit^3</u>

5 0

3 years ago