Phytoplankton is a type of micro algae. This creature is similar to the other plants in the ocean biome. But what makes it special is the fact that it is the main produce in the food web. The plant is so small that it is impossible to see it with the naked eye.

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However, that doesn’t remove the fact that phytoplankton contains a huge amount of chlorophyll. Here are the 13 Function of the Phytoplankton in Ocean Ecosystem. Find out what makes this small plant so crucial to the ocean

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3 years ago

you are running a transformation reaction, and in your microfuge tube currently is your plasmid and your insert fragments (both

ElenaW [278]

A foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction.

<h3>How to explain the reaction?</h3>

With the aid of two enzymes, ligase and restriction enzymes, a foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction. Each enzyme detects a target DNA sequence and cuts it nearby, while ligase aids in connecting the DNA. When two bits of DNA have complimentary bases, it facilitates their joining.

Plasmid and the insert fragment are both present in the microfuge tube, and they both have compatible sticky ends. However, the ligase has been denatured and is no longer active because the prior student left it outside rather than freezing it; despite this, we had already put the ligase into the tube. Ligase aids in binding the plasmid and insert fragments together, but because it is denatured in this instance, it will no longer be able to do so. As a result, no transformation process will take place. And since ligase links DNA fragments together by catalyzing the development of connections between the nearby nucleotides, the two fragments will not be able to unite.

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1 year ago