To run a thin layer chromatography experiment with a chemical substance, begin by marking a horizontal line near the bottom of a
TLC plate with_______. Place a_______spot of the substance onto the line. For the mobile phase, add a small amount of _______ at the bottom of a TLC chamber. Place the plate in, then_______the chamber. Once the mobile phase approaches the top of the plate, remove the plate and mark the _______ line. Note the position of the spots and calculate Rf values if needed.
TLC is thin-layer chromatography, a chromatography technique which is used for separating the non-volatile mixtures.
Explanation:
To run a thin layer thin layer chromatography experiment with a chemical substance, begin by marking a horizontal line near the bottom of TLC plate with PENCIL. Place a SMALL spot of the substance onto the line. For the mobile phase add a small amount of SOLVENT at the bottom of TLC chamber. Place the plate in, then COVER the chamber. Once the mobile phase approaches the top of the plate, remove the plate and mark the SOLVENT line. Note the positions of the spot and calculate the Rf if needed.
A physical change is any change in a substances form that does not change its chemical makeup. Examples of physical changes are breaking a stick or melting ice. A chemical change occurs when atoms of a substance are rearranged, and the bonds between the atoms are broken or formed. HOPE THIS HELPS!!
