0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
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The total number of atoms in 7.10g of chlorine is 1.204 × 10²³atoms.
HOW TO CALCULATE NUMBER OF ATOMS:
- The number of atoms in a substance can be calculated by multiplying the number of moles in that substance by Avogadro's number as follows:
- no. of atoms = no. of moles × 6.02 × 10²³ mol-¹
- The number of moles in 7.10g of Cl is calculated as follows:
no. of moles = mass ÷ molar mass
no. of moles = 7.10g ÷ 35.5g/mol
no. of moles = 0.2mol
no of atoms = 0.2mol × 6.02 × 10²³
no. of atoms = 1.204 × 10²³atoms.
- Therefore, the total number of atoms in 7.10g of chlorine is 1.204 × 10²³atoms.
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Explanation:
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Answer:
200 liters of fluoridated drinking water would have to be consumed by as person to reach the toxic level.
Explanation:
Toxic level of fluoride ions = 0.2 g per 70 kg body weight
Concentration of fluoride ions in drinking water = 1 mg/ L = 0.001 g/L
1 mg = 0.001 g
Let the volume of drinking water consumed by person to reach the toxic level of fluoride concentration be V.
For 70 kg body weight 0.2 grams of fluoride ions will be toxic.
200 liters of fluoridated drinking water would have to be consumed by as person to reach the toxic level.
