Biuret reagent will indicate the presence of protein in a given sample. It is also known as the Piotrowski's test. This reagent consists of copper (II) sulfate and sodium hydroxide. It detects peptide bonds by the reaction of the copper ions in an alkaline solution. The copper ions would form violet colored complexes when peptide is present in the solution. From this test, concentration can be calculated since the intensity of the color depends on the amount of peptide bonds and according to the Beer-Lambert law concentration and the absorption of light is proportional. The concentration is calculated by a spectrophotometric technique at a wavelength of 540 nm.
The phase diagram of CO2 has a melting curve that slopes up and to the right, in contrast to the phase diagram of water, which has a more conventional shape. It is impossible for liquid CO2 to exist at pressures lower than 5.11 atm because the triple point is 5.11 atm and 56.6 °C.
Due to the fact that ice is less thick than liquid water, the phase diagram of water has an odd melting point that drops with pressure. Carbon dioxide cannot exist as a liquid at atmospheric pressure, according to the phase diagram of the gas. Thus, gaseous carbon dioxide directly sublimes from solid carbon dioxide.
Learn more about solid carbon dioxide.
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Answer:
Explanation:
To solve this problem, we need to obtain the number of moles of the solute we desired to prepare;
Number of moles = molarity x volume
Parameters given;
volume of solution = 500mL = 0.5L
molarity of solution = 0.5M
Number of moles = 0.5 x 0.5 = 0.25moles
Now to know the volume stock to take;
Volume of stock = 
molarity of stock = 4M
volume = 
= 0.0625L or 62.5mL
