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aniked [119]
3 years ago
13

What are the primary chemical components present in a phosphate buffer at ph 7.4? H3po4 and po43–?

Chemistry
1 answer:
fenix001 [56]3 years ago
8 0

Answer :

The correct answer for primary component of phosphate buffer at pH = 7.4 is H₂PO₄⁻ and HPO₄²⁻ .

<u>Buffer solution :</u>

It is a solution of mixture of weak acid and its conjugate base OR weak base and its conjugate acid . It resist any change in solution when small amount of strong acid or base is added .

<u>Capacity of a good buffer : </u>

A good buffer is identified when pH = pKa .

From Hasselbalch - Henderson equation which is as follows :

pH = pka + log \frac{[A^-]}{[HA]}

If [A⁻] = [HA] ,

pH = pka + log 1

pH = pKa

This determines that if  concentration of  weak acid  and its conjugate base are changed in small quantity , the capacity of  buffer to maintain a constant pH is greatest at pka .  If the amount of [A⁻] or [HA] is changed in large amount , the log value deviates more than +/-  1M and hence pH .

Hence Buffer has best capacity at pH = pka .

<u>Phosphate Buffer : </u>

Phosphate may have three types of acid-base pairs at different pka ( shown in image ).

Since the question is asking the pH = 7.4

At pH = 7.4 , the best phosphate buffer will have pka near to 7.4 .

If image is checked the acid - base pair  " H₂PO₄⁻ and HPO₄²⁻  has pka 7.2 which is near to pH = 7.4 .

Hence we can say  , the primary chemical component of phosphate buffer at pH = 7.4  is H₂PO₄⁻ and HPO₄²⁻  .


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