One of the best buffer choice for pH = 8.0 is Tris with Ka value of 6.3 x 10^-9.
To support this answer, we first calculate for the pKa value as the negative logarithm of the Ka value:
pKa = -log Ka
For Tris, which is an abbreviation for 2-Amino-2-hydroxymethyl-propane-1,3 -diol and has a Ka value of 6.3 x 10^-9, the pKa is
pKa = -log Ka
= -log (6.3x10^-9)
= 8.2
We know that buffers work best when pH is equal to pKa:
pKa = 8.2 = pH
Therefore Tris would be a best buffer at pH = 8.0.
Plasma is the least common matter on earth
Mass over volume
200 over100
2
Newton’s 2nd law? Maybe I’m not completely sure
