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Andreas93 [3]
3 years ago
12

For the Bradford assay, the instructor will make a Bradford reagent dye by mixing 50 ml of 95% v/v ethanol with 100 mg of Coomas

sie Blue followed by the addition of 100 ml of 85% v/v phosphoric acid. This entire mixture is then diluted to 1 liter with water. What is the final concentration of phosphoric acid?
Chemistry
1 answer:
Flauer [41]3 years ago
4 0

Answer:

4.25% is the final concentration of phosphoric acid.

Explanation:

Initial concentration of phosphoric acid = C_1=85\%=0.85

Initial volume of phosphoric acid = V_1=50 mL

Final concentration of phosphoric acid = C_2=?

Final volume of phosphoric acid = V_2=1 L=1000 mL

( 1L = 1000 mL)

C_1V_1=C_2V_2

C_2=\frac{C_1times V_1}{V_2}

=\frac{0.85\times 50 mL}{1000 mL}=0.0425=4.25%

4.25% is the final concentration of phosphoric acid.

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