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Natasha2012 [34]
3 years ago
13

In a hurry to complete the experiment, Joseph failed to calibrate the spectrophotometer. As a result, all absorbance values for

the standard solutions that are measured and recorded are too high. How will this affect the following for the Test Solutions?
Chemistry
2 answers:
lilavasa [31]3 years ago
7 0

\boxed{{\text{Incorrect results}}} will be observed for test solutions if calibration of spectrophotometer is not done.

Further explanation:

Spectrophotometry

It is a technique that is used to measure the absorbance of light as a measure of the intensity of light when light passes through the sample of the solution. The optical instrument used for such purpose is a spectrophotometer.

Lambert-Beer’s Law

This law relates to the absorbance of the species to its concentration and the optical path length through the sample. The mathematical expression for Lambert-Beer’s law is,

\boxed{A = \varepsilon lc}  

Here,

A is the absorbance of the species.

\varepsilon is the molar absorptivity of the species.

<em>l</em> is the optical path length.

<em>c</em> is the concentration of species.

While using a spectrophotometer, calibration is an essential step in determining the absorbance of the sample. This done by using a blank solution (standard solution) and taking its absorbance value as a reference for other solutions. But if the equipment is not calibrated before measuring the absorbance for other solutions, the results will be incorrect.

In the given experiment, the absorbance values of standard solutions are recorded very high. This will lead to incorrect values of absorbance for test solutions. This is because the absorbance of the standard solution is to be used as a reference for the test solutions.

Learn more:

1. Classify the transitions as absorption or emission of light: brainly.com/question/1594022

2. What is the frequency of yellow light? brainly.com/question/5882803

Answer details:

Grade: Senior School

Subject: Chemistry

Chapter: Beer-Lambert Law

Keywords: absorbance, Lambert-Beer law, calibration, standard solutions, test solutions, blank solution, spectrophotometry, spectrophotometer.

gizmo_the_mogwai [7]3 years ago
5 0
Joseph will not get the correct results for his samples. The spectophotometer will measure wrong absorbance values for the sample. It is highly advised to callibrate the instrument by first setting the absorbance of the solvent to zero. After it is done, only then one must determine the absorbance of the test solutions.
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Full question:

This question is incomplete, here it is completed:

An endergonic reaction with a ______ ∆H and a ______ ∆S can be changed into an exergonic reaction by decreasing the temperature.

Option A: negative, positive

Option B: negative, negative

Option C: positive, positive

Option D: positive, negative

Answer:

Option B: negative, negative

Explanation:

The change in free energy (ΔG) of a system for a constant-temperature process is

ΔG = ΔH - TΔS

free energy is the energy available to do work. Thus, if a particular reaction is accompanied by a release of usable energy (that is, <u>ΔG is negative</u><u>), it is said to be</u><u> exergonic</u>. On the other hand, if a reaction consumes energy (that is, <u>ΔG is positive</u><u>), it is said to be </u><u>endergonic</u>.

Looking at the equation, we can see that if ΔH is negative and ΔS is negative, then ΔG will be negative only when TΔS  is smaller in magnitude than ΔH. This condition is met when T is small.

ΔG = ΔH - TΔS

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This means that the reaction proceeds spontaneously at low temperatures. At high temperatures, the reverse reaction becomes spontaneous. An example of that would be the following reaction:

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Explanation :

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In the galvanic cell, the oxidation occurs at an anode which is a negative electrode and the reduction occurs at the cathode which is a positive electrode.

We are taking the value of standard reduction potential form the standard table.

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The balanced two-half reactions will be,

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Reduction half reaction (Cathode) : Ag^{+}(aq)+e^-\rightarrow Ag(s)

Thus the overall reaction will be,

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