1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Olin [163]
2 years ago
11

Suppose you want to test the results of a transformation by growing Escherichia coli cells in LB medium containing ampicillin as

the antibiotic for selection. Ampicillin at a concentration of 100 µg/mL will kill cells that do not contain an ampicillin resistance gene, but will allow the growth of cells that have been transformed with this gene. The concentrated stock of ampicillin is 100 mg/mL. How many microliters of the ampicillin stock should you add to 50 mL of LB for a bacterial culture?
Chemistry
1 answer:
mestny [16]2 years ago
7 0

Answer:

50.0 μL

Explanation:

When a dilution is done, the mass of the solute (in this case the ampicillin) remains constant, following the Lavoiser's law that the mass is conserved. The mass is the concentration (C) multiplied by the volume (V), so if 1 is the stock solution, and 2 is the bacterial culture after the addition of the antibiotic:

m1 = m2

C1*V1 = C2*V2

C1 = 100 mg/mL = 100000 μg/mL (1 mg = 1,000μg)

C2 = 100 μg/mL

V2 = 50 mL + V1 = 50000μL + V1 (V1 in μL)

100000*V1 = 100*(50000 + V1)

1000V1 = 50000 + V1

999V1 = 50000

V1 = 50.0 μL

You might be interested in
Los electrónes casi no tienen
kondaur [170]

Answer:

yedd

Explanation:

5 0
3 years ago
How many moles of neon gas are there 25.0 L of gas or 5.0°C with a pressure of 89.9 KPa?
vladimir2022 [97]
  • Volume=25L
  • Temperature=5°C
  • Pressure=89.9kPa

Now

\\ \rm\rightarrowtail PV=nRT

\\ \rm\rightarrowtail n=\dfrac{PV}{RT}

\\ \rm\rightarrowtail n=\dfrac{89.9(25)}{5R}

\\ \rm\rightarrowtail n=89.9(5)/R

\\ \rm\rightarrowtail n=449.5/R mol

8 0
2 years ago
PLZZZZZZZ HELPPPPPPPPPPP:))))
MArishka [77]
Answer:

C

Explanation:

Smooth muscles are in the digestive system, skeletal muscles are the ones we voluntarily move, and cardiac muscles are for the heart.
7 0
2 years ago
FILL IN TH BLANK PLEASES 8TH GRADE SCIENCE!!!!
asambeis [7]

The elements helium neon and Exon are all part of the same _______ on the periodic table.


B. Group

5 0
3 years ago
How are plant and animal cells similar? How are they different? To answer these questions, make a list of the different organell
aleksley [76]

Answer:

A plant cell contains a large, singular vacuole that is used for storage and maintaining the shape of the cell. In contrast, animal cells have many, smaller vacuoles. Plant cells have a cell wall, as well as a cell membrane. In plants, the cell wall surrounds the cell membrane.

7 0
2 years ago
Other questions:
  • Carbon, hydrogen, silicon, nitrogen, sulfur, and phosphorus are the six most important elements found in living things.
    11·1 answer
  • Describe at least two experiments, including the observed results, that caused the scientific community to accept changes to Dal
    6·1 answer
  • The standard metric units of density are:<br> kg/N<br> g/cm3<br> kg/cm3<br> g/L
    6·2 answers
  • im found only in plant cells im as green as can be i make food for the plant using the suns energy what am i?
    11·2 answers
  • 11. Power can be defined as (2 points)
    5·1 answer
  • Ted used a balance scale to weigh a 2.233 g sample of copper sulfate. Which of these measurements made by Ted is the most accura
    6·1 answer
  • Which drug presents the greatest challenges to forensic science
    5·1 answer
  • Can someone please help!
    15·1 answer
  • an atom of which element has the strongest attraction for electons in a chemical bond?cholorine, phosphorus, carbon, sulfur
    12·1 answer
  • Sierra has a special kind of liquid rubber. She knows that ultraviolet light is
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!