Thin layer chromatography(TLC) works with the principle of separation through adsorption.
It is used in the isolation and extraction of lipids through the following steps:
- apply the lipid samples spots in the bottom of the plate.
- also apply sample solution to the marked spot
- pour the mobile phase into the TLC chamber and use a moist filter paper to cover it. this is done to maintain equal humidity.
- then place the plate in the TLC chamber and close it with a lid.
- the plate is immersed into the solvent (mobile phase) for its development. this is done, keeping in mind that the sample spot should be above the solvent.
- once the sample spots are developed, they are removed and dried.
- this is later viewed using the UV light chamber to see the isolation of the lipid sample.
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<span>The solubility of solutes is dependent on temperature. When a solid dissolves in a liquid, a change in the physical state of the solid analogous to melting takes place. Heat is required to break the bonds holding the molecules in the solid together. At the same time, heat is given off during the formation of new solute -- solvent bonds.</span>
Answer:
F centripetal force (tension) = 275.9 N
Explanation:
Given data:
Mass = 1.50 kg
Radius = 0.520 m
Velocity of ball = 9.78 m/s
Tension = ?
Solution:
F centripetal force (tension) = m.v² / R
F centripetal force (tension) = 1.50 kg . (9.78 m/s)² / 0.520 m
F centripetal force (tension) = 1.50 kg . 95.65 m²/s² / 0.520 m
F centripetal force (tension) = 143.5 kg. m²/s² / 0.520 m
F centripetal force (tension) = 275.9 N