For an approximate result, multiply the volume value by 3.785
Answer ≈ 56.7812
Hey there!:
From the given data ;
Reaction volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )
Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:
[E]t in molar = g/L * mol/g
[E]t = 0.01 g/L * 1 / 45,000
[E]t = 2.22*10⁻⁷
Vmax = 0.758 umole/min/ per mL
= 758 mmole/L/min
=758000 mole/L/min => 758000 M
Therefore :
Kcat = Vmax/ [E]t
Kcat = 758000 / 2.2*10⁻⁷ M
Kcat = 3.41441 *10¹² / min
Kcat = 3.41441*10¹² / 60 per sec
Kcat = 5.7*10¹⁰ s⁻¹
Hence kcat of xyzase is 5.7*10¹⁰ s⁻¹
Hope that helps!
7 valance electrons and a atomic number of 9
<span>THE HIGHEST CONCENTRATION OF HYDROGEN IONS IS LOCATED IN THE INTER-MEMBRANE SPACE. HYDROGEN IONS REACH THE INTER-MEMBRANE SPACE THROUGH PROTEIN CHANNELS EMBEDDED IN THE MITOCHONDRIAL MEMBRANE. THE MAIN FUNCTION OF INTER MEMBRANEIS OXIDATIVE PHOSPHORLATON. ENERGY IS REQUIRED TO MOVE THE HYDROGEN IONS ACROSS THE MEMBRANE BECAUSE THE HYDROGEN IONS ARE MOVING AGAINST THE CONCENTRATION GRADIENT. H+ GOES AGAINST THE CONCENTRATION GRADIENT THE USE OF THE GRADIENT TO DRIVE ATP SYNTHASE. HYDOGEN IONS DRIVE ATP SYNTHASE IN PHTOSYNTHESIS. THIS HAPPENS WHEN HYDROGEN IONS GET PUSHED ACROSS THE MEMBRANE CREATING A HIGH CONCENTRATION INSIDE THE THYLAKOID.</span>
